mouse il 6 elisa kit Search Results


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Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via <t>ELISA</t> are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.
Mouse Il 6 Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via <t>ELISA</t> are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.
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Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via <t>ELISA</t> are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.
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Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via <t>ELISA</t> are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.
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Elabscience Biotechnology mouse il 6 elisa kit
Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via <t>ELISA</t> are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.
Mouse Il 6 Elisa Kit, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via <t>ELISA</t> are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.
Il 6, supplied by Boster Bio, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via <t>ELISA</t> are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.
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Image Search Results


Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via ELISA are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.

Journal: JCI Insight

Article Title: Quinolinic acid potentially links kidney injury to brain toxicity

doi: 10.1172/jci.insight.180229

Figure Lengend Snippet: Kidney cortex RNA and protein lysates were prepared from Mdm2 -cKO vs. control mice ( n = 4 per group). ( A ) Transformation related protein 53 ( Trp53 ) mRNA and ( B ) p53 protein levels. ( C ) Cyclin-dependent kinase inhibitor 1A ( Cdkn1a ) mRNA and ( D ) p21 protein levels. ( E ) Cytochrome b-245, beta polypeptide ( Cybb ), mRNA and ( F ) CYBB protein levels. ( G ) CXC chemokine ligand 1 ( Cxcl1 ) mRNA level. ( H ) CXCL1 protein concentration. ( I ) Interleukin-1β ( Il1b ) mRNA level. ( J ) IL-1β protein concentration. ( K ) Neutrophil-to-lymphocyte ratio (VetScan Analyzer). ( L ) Plasma interleukin-6 (IL-6) protein concentration. ( M ) Plasma soluble urokinase plasminogen activator receptor (suPAR) concentration. Quantitative PCR (qPCR) results (normalized to Gapdh expression) and protein levels measured via Western blot (normalized to actin) are presented as fold-change relative to control. The proteins analyzed via ELISA are reported as concentrations. Graphs display means ± SEM. Two-tailed t tests: * P < 0.05, ** P < 0.01, and *** P < 0.001.

Article Snippet: Quantitative protein levels in plasma and tissue lysates were assessed using ELISA kits following manufacturer’s protocols: mouse uPAR DuoSet ELISA (R&D Systems, Bio-Techne, DY531), mouse CXCL1/KC Quantikine ELISA Kit (R&D Systems, Bio-Techne, MKC00B-1), mouse IL-6 Quantikine ELISA Kit (R&D Systems, Bio-Techne, M6000B-1), and mouse IL-1β/IL-1F2 Quantikine ELISA Kit (R&D Systems, Bio-Techne, MLB00C-1).

Techniques: Control, Transformation Assay, Protein Concentration, Clinical Proteomics, Concentration Assay, Real-time Polymerase Chain Reaction, Expressing, Western Blot, Enzyme-linked Immunosorbent Assay, Two Tailed Test

Mice were fed regular chow (control) or 0.2% adenine-enriched chow for 1 month ( n = 4 per group). ( A and B ) Plasma suPAR and IL-6 levels measured by ELISA. Concentrations of Trp, KYN, 3HK, QA, and 5-HIAA in ( C – G ) plasma, kidney cortex ( H – L ), and brain cortex ( M – Q ). QA and 5-HIAA were measured using a different mass spectrometry method. Graphs display means ± SEM. Two-tailed t test; * P < 0.05, ** P < 0.01, *** P < 0.001.

Journal: JCI Insight

Article Title: Quinolinic acid potentially links kidney injury to brain toxicity

doi: 10.1172/jci.insight.180229

Figure Lengend Snippet: Mice were fed regular chow (control) or 0.2% adenine-enriched chow for 1 month ( n = 4 per group). ( A and B ) Plasma suPAR and IL-6 levels measured by ELISA. Concentrations of Trp, KYN, 3HK, QA, and 5-HIAA in ( C – G ) plasma, kidney cortex ( H – L ), and brain cortex ( M – Q ). QA and 5-HIAA were measured using a different mass spectrometry method. Graphs display means ± SEM. Two-tailed t test; * P < 0.05, ** P < 0.01, *** P < 0.001.

Article Snippet: Quantitative protein levels in plasma and tissue lysates were assessed using ELISA kits following manufacturer’s protocols: mouse uPAR DuoSet ELISA (R&D Systems, Bio-Techne, DY531), mouse CXCL1/KC Quantikine ELISA Kit (R&D Systems, Bio-Techne, MKC00B-1), mouse IL-6 Quantikine ELISA Kit (R&D Systems, Bio-Techne, M6000B-1), and mouse IL-1β/IL-1F2 Quantikine ELISA Kit (R&D Systems, Bio-Techne, MLB00C-1).

Techniques: Control, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Mass Spectrometry, Two Tailed Test